Fig. 3: The activity assay and physiological function of SpCmoX in vitro and in vivo. | Nature Communications

Fig. 3: The activity assay and physiological function of SpCmoX in vitro and in vivo.

From: Carboxymethylcytosine is a natural base modification and a handle for bacteriophage DNA hypermodification

Fig. 3: The activity assay and physiological function of SpCmoX in vitro and in vivo.

a LC–MS extracted ion chromatogram of the digested dsDNA product, monitoring the formation of 5cxmdC (m/z = 286.1). b ESI (+) m/z spectrum corresponding to the EIC peak for 5cxmdC. c Agarose gel analysis of HhaI digestion of the SpCmoX-treated dsDNA. This experiment was independently repeated three times with consistent results. d LC–MS extracted ion chromatograms detecting the presence of 5cxmdC (m/z = 286.1) from digested HT-SpcmoX plasmids extracted from BL21 (DE3) and BL21 (DE3) ΔcmoA cells. e The ESI (+) m/z spectrum corresponding to EIC peak for 5cxmdC.

Back to article page