Fig. 4: Nanopore sequencing of Synechococcus phage S-B43 genome and sequence specificity for SpCmoX. | Nature Communications

Fig. 4: Nanopore sequencing of Synechococcus phage S-B43 genome and sequence specificity for SpCmoX.

From: Carboxymethylcytosine is a natural base modification and a handle for bacteriophage DNA hypermodification

Fig. 4: Nanopore sequencing of Synechococcus phage S-B43 genome and sequence specificity for SpCmoX.

a Plot of the estimation results of the relative position of the modified base in the enriched motif. b Motif predicted by MEME from significantly modified regions of the Synechococcus phage S-B43 genome. To facilitate motif discovery, five positions on either side of the most modified base per region were included. c LC–MS extracted ion chromatograms monitoring the formation of 5cxmdC (m/z = 286.1) in various assays. The substrate dsDNA was prepared by annealing 25 bp palindromic oligonucleotides (Supplementary Table 3). In the enzyme-free negative controls, GCG(T/G/C) and (G/C/A)GCG, mixed annealed oligos of the three preceding assays, were used as substrates. d ESI (+) m/z spectrum corresponding to EIC peak for 5cxmdC.

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