Fig. 6: The activity assay of SpCmoY. | Nature Communications

Fig. 6: The activity assay of SpCmoY.

From: Carboxymethylcytosine is a natural base modification and a handle for bacteriophage DNA hypermodification

Fig. 6: The activity assay of SpCmoY.The alt text for this image may have been generated using AI.

a Extraction of the genomic DNA from E. coli cells overexpressing SpCmoX. b Extraction of the genomic DNA from E. coli cells overexpressing both SpCmoX and SpCmoY. c LC–MS extracted ion chromatograms monitoring the formation of 5cxmdC (m/z = 286.1) and 5gcxmdC (m/z = 343.1) in the genomic DNA of (a). d LC–MS extracted ion chromatograms monitoring the formation of 5cxmdC (m/z = 286.1) and 5gcxmdC (m/z = 343.1) in the genomic DNA of (b). e ESI (+) m/z spectrum corresponding to EIC peak for 5cxmdC in (c). f ESI (+) m/z spectrum corresponding to EIC peak for 5gcxmdC in (d). g In vitro SpCmoY enzyme activity assay with the genomic DNA of (a) as the substrate. LC–MS/MS extracted ion chromatograms were used to monitor the formation of 5gcxmdC (m/z = 343.1 → 227.1). h Proposed model for the SpCmoY-catalyzed reaction.

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