Fig. 3: CD44 cross-linking induces phosphorylation of RBC cytoskeletal proteins.
From: CD44 cross-linking promotes Plasmodium falciparum invasion
RBCs were stimulated with BRIC 222 or BRIC 222 F(ab) at 25 µg/ml, processed into ghosts, and used for 2D-DIGE. a Total Protein and Phosphoprotein gels, along with the overlays of each condition are shown. Spots with differential phosphorylation, with ≥1.5 or ≤0.67 fold-change in phosphorylation, between the BRIC 222 F(ab)- and BRIC 222-stimulated conditions are circled. White circles indicate higher phosphorylation in BRIC 222-stimulated samples; blue circles indicate higher phosphorylation in BRIC 222 F(ab)-stimulated samples. b Top phosphorylated candidates, numbered in (a) with their respective phospho-volume. Adjusted phospho-ratio indicates phosphorylation ratio adjusted based on protein expression. The candidate proteins were identified by mass spectrometry, with the associated gene indicated in the parentheses.
