Fig. 3: PBGProOx20 exerts bactericidal activity by destroying bacterial membranes. | Nature Communications

Fig. 3: PBGProOx20 exerts bactericidal activity by destroying bacterial membranes.

From: Biguanide-functionalized peptide mimics effectively combat drug-resistant ESKAPE pathogens and meningitis

Fig. 3

Time-laps confocal images of S. aureus (a) and E. coli (b) at the presence of dye-PBGProOx20 (green, 2Ă—MIC) and PI (red, 10 μg/mL). c Fluorescence intensity of dye-PBGProOx20 and PI across S. aureus at 1 min and 17 min. d Fluorescence intensity of dye-PBGProOx20 and PI across E. coli at 1 min and 19 min. e The changes of intracellular mean fluorescent intensity with incubation time of dye-PBGProOx20 and PI on S. aureus and E. coli. f Confocal images of FM4-64 stained S. aureus and E. coli after treatment with PBGProOx20 (2Ă—MIC) for 2 h. g Fluorescence changes of SYTOX in S. aureus and E. coli after treatment with PBGProOx20 at different concentrations (n = 3). Data are presented as mean ± SD. SEM (h) and TEM (i) characterization of S. aureus and E. coli before (the controls) and after treatment with PBGProOx20 (2Ă—MIC) for 2 h.

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