Fig. 4: FLICK-PE mediates efficient intended editing in soybean stable transformation.

Percentages of intended editing, unintended editing and indels at target-site 1 (PE2 n = 25, PE3 n = 23, FLICK-PE n = 19) (a), target-site 3 (PE2 n = 21, PE3 n = 30, FLICK-PE n = 27) (b) and target-site 5 (PE2 n = 27, PE3 n = 32, FLICK-PE n = 25) (c) induced in T₀ soybean stable transformants by PE2, PE3 and FLICK-PE, as determined via Hi-TOM deep sequencing. d The effective intended-editing efficiency of PE2, PE3 and FLICK-PE in T₀ soybean stable transformants. The effective intended-editing events were defined as Hi-TOM deep sequencing-validated intended editing rates ≥ 20%. Sanger sequencing chromatograms of two T₀ lines carrying effective intended edits (indicated by red arrowheads) at target-site 1 (e), target-site 3 (f) and target-site 5 (g) induced by FLICK-PE. The central black lines in the violin plots represent the median in (a–c). The horizontal gray lines represent the lower and upper quartiles, and the shaded areas represent data-distribution density (a–c). The “intended edit reads” above the chromatograms indicate the proportion of intended edits in this line detected via Hi-TOM sequencing (e–g). Source data are provided as a Source data file.