Fig. 2: ZnF domains 1 and 2 regulate nick binding. | Nature Communications

Fig. 2: ZnF domains 1 and 2 regulate nick binding.

From: Nucleosome unwrapping and PARP1 allostery drive affinities for chromatin and DNA breaks

Fig. 2: ZnF domains 1 and 2 regulate nick binding.

a A representative kymograph (white lines indicate PARP1 binding events) of PARP1 ZnF1-2 binding nicked lambda DNA. Resultant CRTD and CGTD plots are shown below, as well as the measured KD. b PARP2 engages nick sites (white lines represent binding). Off rate and on rate measurements are shown below, as well as resultant KD. Created in BioRender. Schaich, M. (2025). https://BioRender.com/ufjd01j.

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