Fig. 6: The role of PARP1 allostery on DNA nick and nucleosome binding kinetics.
From: Nucleosome unwrapping and PARP1 allostery drive affinities for chromatin and DNA breaks
a A representative kymograph of catalytically-dead PARP1 binding nicked DNA, with several events lasting over 100 s (white lines). CRTD and CGTD plots are also shown. b With 100 µM EB-47 present (which saturates the protein to > 99% bound), WT-PARP1 events last much longer (white lines), and c with 100 µM Olaparib included. Determination of on and off rates are shown below, as well as the calculated KD for the interaction. d Binding kinetics and resultant CRTD/CGTD plots for catalytically-dead PARP1 and e inhibited PARP1 on undamaged nucleosomes. With each CRTD plot, the fit for uninhibited WT PARP1 on nicked DNA is shown as a dotted red line for reference (τ = 2.3 s). Created in BioRender. Schaich, M. (2025). https://BioRender.com/oml86m7.
