Fig. 7: Investigating C-terminal truncations of the geminin coiled-coil.
From: Geminin inhibits DNA replication licensing by sterically blocking CDT1-MCM2 interactions
a Alignment of the AlphaFold prediction of CDT1 (white) in complex with geminin (shown in cartoon form with tubular helices to highlight the C-terminus of the coiled-coil), with the human OCCM structure (PDB ID 8RWV). When aligning CDT1 in the AlphaFold structure with CDT1 in the human OCCM the steric clash between geminin and MCM2 (peach, shown in transparent surface view) is evident. Truncating the C-terminus of the geminin mimetic peptide reduces this clash. Figure made using Chimera X. b Cartoon of geminin peptides Gem76-145, Gem76–140, or Gem76–135. Created in BioRender. Faull, S. (2025) https://BioRender.com/3k0eyee. c Kinetic and dissociation constants for SPR experiments, n = 3, shown as mean ± SE. d Plot of KD values for binding of geminin peptides and immobilised CDT1ΔN from SPR experiments. Data shown as the mean of 3 independent repeats ±SE. e Pre-RC HS-washed elutions after addition of GemFL, Gem76-145, Gem76–140 or Gem76–135 to the pre-RC reaction. f Quantification of the mean MCM2-7 band intensity for reactions shown in (e) normalised to the control pre-RC reaction and compared mixed-effects analysis with Tukey’s multiple comparisons test. ****p < 0.0001. Data shown as the mean ± SD. Source data are provided as a Source Data file.
