Fig. 6: Knocking down HOXB9 and ODC1 inhibits tumor formation and LNM in HFD-induced EC mice models.
From: Metabolic syndrome promotes endometrial cancer by Oleic acid-mediated polyamine accumulation
a Schematic workflow of the animal experiment (created with Figdraw; https://www.figdraw.com/). b Serum levels of total triglycerides (TG), total cholesterol (TC), glucose, HDL, and LDL in subcutaneous tumor model mice (sh group) after 2-month diet induction [high-fat-diet (HFD): n = 3, low-fat-diet (LFD): n = 3)]. Data: mean ± SD; two-tailed student’s t-test. c Representative MRI images of renal capsular fat tissue from lymph node metastasis (LNM) model mice (HFD: n = 4, LFD: n = 4) after 2-month diet induction. d Quantitative statistics of whole-body adipose tissue volume in MRI-scanned mice from (c). Data: mean ± SD (HFD: n = 4, LFD: n = 4); two-tailed student’s t-test. e 3 × 10⁶ Ishikawa cells (stable expressing shNC/shHOXB9/shODC1) were inoculated into the axilla of ovariectomized nude mice. Xenograft tumors were dissected and photographed 24 days post-inoculation. f Tumor growth curves (subcutaneous model, sh group: HFD-NC n = 5, others n = 4 per group). Data: mean ± SD; two-tailed one-way ANOVA. g Xenograft tumor weights [same group sizes as (f)]. Data: mean ± SD; two-tailed one-way ANOVA. h Serum BzCl-derivatized putrescine in subcutaneous model [same group sizes as (f)], measured by LC-MS. Data: mean ± SD; two-tailed one-way ANOVA. i Serum BzCl-derivatized spermidine in subcutaneous model [same group sizes as (f)], measured by LC-MS. Data: mean ± SD; two-tailed one-way ANOVA. j BzCl-derivatized putrescine in xenograft tissues [same group sizes as (f)], measured by LC-MS (0 for no tumor). Data: mean ± SD; two-tailed one-way ANOVA. k Correlation between tissue putrescine response area and tumor volume; two-tailed Pearson correlation. l 3 × 10⁶ Ishikawa cells (stable expressing sgNC/sgHOXB9-exon1/sgODC1-exon4) were inoculated into ovariectomized nude mice. sgNC + DFMO group: 1% (w/v) DFMO in drinking water when tumors were palpable. Tumors dissected/photographed 30 days post-inoculation: n = 6 per group. m 5 × 10⁶ luciferase-tagged AN3CA cells (stable expressing shNC/shHOXB9/shODC1) were inoculated into footpad of ovariectomized mice. shNC+DFMO group: 1% (w/v) DFMO in drinking water 2 months post-inoculation. Luciferase signals measured by IVIS Spectrum 3 months post-inoculation; n = 5 per group. n Quantification of lymph node metastasis signals [Average Radiance (photons per second per square centimeter per steradian (p/s/cm²/sr)] from (m). Data: mean ± SD; two-tailed one-way ANOVA; n = 5 per group. Multiple comparisons were corrected. Source data are provided.
