Table 3 Antibodies

From: HIF-1α-mediated feedback prevents TOR signalling from depleting oxygen supply and triggering stress during normal development

Antibody

Host

Source

Concentration

V5

Mouse

Thermo Fisher Scientific, R960-25, RRID: AB_2556564

1:500

PAb2627

Rabbit

Hypoxyprobe, Inc., HP PAb2627

1:200

pS6

Rabbit

A gift from Jongkyeong Chung, Ref. 111

1:5000

pS6

Rabbit

A gift from Felipe Karam Teixeira, Ref. 112

1:500

pS6

Rabbit

This study

1:100

S6 (54D2)

Mouse

Cell Signalling Technology, 2317, RRID:AB_2238583

1:100

Ci

Rat

DSHB, 2A1, RRID:AB_2109711

1:100

β-Tubulin

Mouse

DSHB, E7, RRID:AB_528499

1:500

Alexa FluorTM Plus 647, anti-mouse

Goat

Thermo Fisher Scientific, A32728, RRID: AB_2633277

1:1000

Alexa FluorTM Plus 555, anti-rabbit

Goat

Thermo Fisher Scientific, A32732, RRID:AB_2633281

1:1000

IRDye® 800CW anti-rabbit IgG

Donkey

LI-COR Biotechnology, 926-32213, RRID:AB_621848

1:10,000

IRDye® 680RD anti-mouse IgG

Donkey

LI-COR Biotechnology, 926-68070, RRID:AB_10956588

1:10,000

Alexa FluorTM 647, anti-rat

Goat

Thermo Fisher Scientific, A-21247, RRID:AB_141778

1:1000