Table 3 Antibodies
Antibody | Host | Source | Concentration |
|---|---|---|---|
V5 | Mouse | Thermo Fisher Scientific, R960-25, RRID: AB_2556564 | 1:500 |
PAb2627 | Rabbit | Hypoxyprobe, Inc., HP PAb2627 | 1:200 |
pS6 | Rabbit | A gift from Jongkyeong Chung, Ref. 111 | 1:5000 |
pS6 | Rabbit | A gift from Felipe Karam Teixeira, Ref. 112 | 1:500 |
pS6 | Rabbit | This study | 1:100 |
S6 (54D2) | Mouse | Cell Signalling Technology, 2317, RRID:AB_2238583 | 1:100 |
Ci | Rat | DSHB, 2A1, RRID:AB_2109711 | 1:100 |
β-Tubulin | Mouse | DSHB, E7, RRID:AB_528499 | 1:500 |
Alexa FluorTM Plus 647, anti-mouse | Goat | Thermo Fisher Scientific, A32728, RRID: AB_2633277 | 1:1000 |
Alexa FluorTM Plus 555, anti-rabbit | Goat | Thermo Fisher Scientific, A32732, RRID:AB_2633281 | 1:1000 |
IRDye® 800CW anti-rabbit IgG | Donkey | LI-COR Biotechnology, 926-32213, RRID:AB_621848 | 1:10,000 |
IRDye® 680RD anti-mouse IgG | Donkey | LI-COR Biotechnology, 926-68070, RRID:AB_10956588 | 1:10,000 |
Alexa FluorTM 647, anti-rat | Goat | Thermo Fisher Scientific, A-21247, RRID:AB_141778 | 1:1000 |