Fig. 5: In vitro cellular uptake and pyroptosis. | Nature Communications

Fig. 5: In vitro cellular uptake and pyroptosis.

From: Constructing flexible sub-nanometer ferroelectric catalyst to overcome heterocatalytic kinetic barriers for enhanced catalytic and immuno-therapy

Fig. 5: In vitro cellular uptake and pyroptosis.

a Schematic diagram of THZO NWs mediated cooperative piezoelectric catalysis, enzyme catalysis and pyroptosis. CLSM images of FITC-labeled THZO NWs co-located with b lysosomes and c mitochondria. Bio-TEM images of 4T1 cells incubated with THZO NWs for d 0 h, e 1 h, and f 4 h. g Bio-TEM image of 4T1 cells after US treatment. Red and blue arrows locate THZO NWs in lysosomes and mitochondria, respectively. h Associated mean fluorescence intensity of intracellular ROS level under various conditions. Blue, DAPI, Green, DCFH-DA. i Digital images and k corresponding quantitative analysis of 4T1 cell scratching tests. Data are presented as mean ± S.D. (n = 3 biologically independent cell samples). j Respective images and l corresponding quantification analysis for the colony formation of 4T1 cells upon the indicated treatments. Data are presented as mean ± S.D. (n = 3 biologically independent cell samples). m 4T1 multicellular spheroids stained with Calcein-AM/PI. n Flow cytometry analysis of 4T1 cells and o corresponding quantitative analysis of cell apoptosis percentages under various conditions. p Expression of HMGB1 and CRT in 4T1 cells under different conditions. q Western blotting analysis of Caspase-1, C-Caspase-1, GSDMD, N-GSDMD, and NLRP3 expression. r Flow cytometry analysis of stimulated maturation state of DCs after different treatments.

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