Fig. 1: Mouse intelectin-2 (mItln2) is conditionally expressed in type 2 inflammation.

A Relative quantification (mean ± SEM) of total Itln expression in enteroids derived from B6.C-Itln1-6 mice after treatment with different cytokines. Goblet cell product Clca1 and intestinal epithelial cell product Reg3g served as positive controls for IL-4/ IL-13 and IL-22 treatments, respectively (collective data from 3 independent experiments). B Relative quantification (mean ± SEM) of Itln1 expression in enteroids derived from C57BL/6 N mice after treatment with different cytokines (collective data from 3 independent experiments). (C, D) Percentage of total Itln sequencing reads mapped to different Itln paralogs. Data represent percent reads from bulk RNAseq analysis of pooled samples from distal small intestine in B6.C-Itln1-6 mice (n = 3) (C) and B6.C-Itln1-6-derived enteroids stimulated with different cytokines (n = 6 independent experiments) (D). E Schematic of mItln2 expression in mouse lungs and intestines upon exposure to helminths and allergens. Created in BioRender. Lab01, K. (2025) https://BioRender.com/6ylynqc. Statistical analysis in (A) and (B) involves one-way ANOVA with Dunnett’s multiple comparisons test. Box and whisker plots show all data points with center line representing the median, the box is the interquartile range (Q1–Q3), and whiskers extend to absolute maximum and minimum values in the dataset. For complete data, see the source data file.