Fig. 5: MgATP induced ε-subunit rearrangements in P. aeruginosa ATP synthase.

Cryo-EM analysis of P. aeruginosa ATP synthase after incubation with 10 mM MgATP yielded multiple maps, capturing the enzyme in distinct rotary and inhibitory states (Fig. S7). Two maps of the enzyme in rotary State 2 are shown here, illustrating different conformations of the inhibitory ε subunit. Left: autoinhibited “up” extended state, preventing rotation. Right: active “down” condensed state, allowing the rotation of the γ subunit. Cryo-EM maps colored as in previous figures, with non-protein density transparent, and close-up views of the εCTD with helices labelled.