Fig. 1: The generation of the native Otof c.1315 C > T (p.R439*) mouse model. | Nature Communications

Fig. 1: The generation of the native Otof c.1315 C > T (p.R439*) mouse model.

From: CRISPR-free RNA base editing mediated PTC-readthrough restores hearing in mice with Otof nonsense mutation

Fig. 1: The generation of the native Otof c.1315 C > T (p.R439*) mouse model.

a sgRNA design to construct the Otof c.1315 C > T (p.R439*) mouse model. Otof-targeted sgRNA contains a complementary C pairing with the 1315 C > T located in exon 14 of the Otof gene. b Sanger sequencing indicated the success of the construction of Otof c.1315 C > T (p.R439*) mouse model. c Homology alignment analysis between Mus (Mouse) and Homo (Human) Otof transcripts V1, and the frame showing the 100% homology region that includes the site of Otof c.1315 C > T. The marked c base corresponds to position c.1315 in the mouse Otof gene and c.1273 in the human OTOF gene. CDS: Coding Sequence. UTR: Untranslated Region.

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