Fig. 2: Functional and immunostained evaluation of Otof c.1315 C > T (p.R439*) mice. | Nature Communications

Fig. 2: Functional and immunostained evaluation of Otof c.1315 C > T (p.R439*) mice.

From: CRISPR-free RNA base editing mediated PTC-readthrough restores hearing in mice with Otof nonsense mutation

Fig. 2: Functional and immunostained evaluation of Otof c.1315 C > T (p.R439*) mice.

a No ABR waveforms were elicited in the Otof c.1315 C > T (p.R439*) mice compared with the WT mice. b Statistical analysis of ABR thresholds between the Otof c.1315 C > T (p.R439*) (n = 3 biological samples) and WT mice (n = 6 biological samples), significant elevation of ABR thresholds was seen in the Otof c.1315 C > T (p.R439*) mice compared with the WT mice. Data are represented as the mean ± SD. c A schematic diagram for the harvest of cochlear basilar membrane. d The whole mount staining showed absence of otoferlin expression in the Otof c.1315 C > T (p.R439*) mice, whereas there was normal otoferlin expression in the WT mice. The white frames indicate the enlarged region of IHCs. (Myosin Ⅶa, red; otoferlin, green; DAPI, blue.). Scale Bars = 200 μm, 50 μm, 10 μm. This experiment was conducted with three independent replicates. IHCs: inner hair cells; OHCs: outer hair cells. Source data are provided as a Source Data file.

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