Fig. 1: Schematic diagram of TamAB.
From: Structural basis of outer membrane biogenesis by the TamAB translocase
a TamA contains three POTRA domains in the periplasm and a 16-stranded beta-barrel in the OM with eight extracellular loops (ECL1-8) and seven periplasmic loop (PL1-7). b TamB consists of an N-terminal inner membrane helix, a periplasmic beta-taco domain, and a C-terminal domain formed by two helices and six beta-strands with five loops (LP1-5). c Schematic representation of TamAB. TamAB forms a trans-envelope nanomachine, transporting phospholipid from the inner membrane to the outer membrane and assembling autotransporter proteins in the outer membrane. d Size-exclusion chromatography of purified TamAB. e SDS-polyacrylamide gel electrophoresis of purified TamAB. All experiments were repeated at least three times. Source data are provided as a Source Data file.
