Fig. 2: Evaluation of the percentage of AAV-Ant1 transduced cells. | Nature Communications

Fig. 2: Evaluation of the percentage of AAV-Ant1 transduced cells.

From: Partial restoration of mitochondrial dysfunction by AAV-Ant1 protects from dilated cardiomyopathy in Ant1-/- plus mtDNA mutant mice

Fig. 2: Evaluation of the percentage of AAV-Ant1 transduced cells.The alternative text for this image may have been generated using AI.

A Representative section of one Ant1+/+ AAV-Ant1 transduced heart stained with Hoechst for nuclei (blue), WGA to outline cells (red), and GFP (green). Successfully transduced cardiomyocytes are surrounded by red, with the cytoplasm stained green and nuclei blue. B Quantification of the percentage of GFP-positive cardiomyocytes, with each dot representing data from one microscopic field. Three sections from each heart, representing different anatomical planes (coronal, anterior, and posterior), were analyzed, with five random fields examined per section across three hearts, totaling 5 × 3 × 3 = 45 random fields. Center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range. An average of ~20% of cardiomyocytes are GFP-positive and thus transduced. C Representative images of GFP immunohistochemical staining intensity, indicating overall ventricular GFP expression. D Quantification of the relative ventricular area with GFP expression in AAV-Ant1 transduced hearts at 1, 3, 6, 9, and 12 months. Each point represents a mouse (WT = black dots, Ant1−/− = red dots, and Ant1−/−+ND6P25L = blue dots; Median is indicated; n = 2 per condition), with individual values reported in the table.

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