Fig. 5: Gene expression and proteomic analysis of AAV-ANT1 transduction of Ant1+/+, Ant1−/−, Ant1−/−+ND6P25L mouse hearts. | Nature Communications

Fig. 5: Gene expression and proteomic analysis of AAV-ANT1 transduction of Ant1+/+, Ant1−/−, Ant1−/−+ND6P25L mouse hearts.

From: Partial restoration of mitochondrial dysfunction by AAV-Ant1 protects from dilated cardiomyopathy in Ant1-/- plus mtDNA mutant mice

Fig. 5: Gene expression and proteomic analysis of AAV-ANT1 transduction of Ant1+/+, Ant1−/−, Ant1−/−+ND6P25L mouse hearts.The alternative text for this image may have been generated using AI.

A The Ant1 mRNA level in WT = Ant1+/+, Ant1−/−, and Ant1−/−+ND6P25L heart samples injected with AAV2/9-pDes-Gfp-mAnt1 vector (AAV-Ant1) or PBS (n = 3, mean); B fold change (AAV-Ant1) (purple column) compared with PBS-injected mice (turquoise column); C and D Normalized Enrichment Score analysis of extracellular matrix (ECM) and sarcomere proteins expression in Ant1−/− and Ant1−/−+ND6P25L heart samples injected with AAV2/9-pDes-Gfp-mAnt1 vector (AAV-Ant1) compared with PBS-injected mice (Ant1−/−+AAV-Ant1 virus-injected, purple column; Ant1−/− PBS injected, turquoise column; Ant1−/−+ND6P25L AAV-Ant1 virus injected, blue column; Ant1−/−+ND6P25L PBS injected, orange column); E and F ANT1 and ANT2 levels assessed by mass spectrometry in isolated hearts from WT = Ant1+/+ and Ant1−/− mice transduced with AAV2/9-pDes-Gfp-mAnt1 vector (AAV-Ant1) or non-transduced Ant1−/−. E ANT1 single peptides (gray lines) and ANT1 protein (brown dash line), F, ANT2 single peptides (gray lines) and ANT2 protein (brown dash line); G–I Volcano plot of the mass spectrometry data obtained comparing Ant1−/− versus Ant1+/+ mice (G), Ant1−/− AAV-Ant1 vector injected versus Ant1−/− mice (H), Ant1 peptides were observed but fell below Log2 fold change scale range shown), and Ant1−/− AAV-Ant1 vector transduced versus Ant1+/+ mice (I). Three animals per condition were evaluated for RNA-seq and mass spectrometry studies. Statistic methods used are: DESeq2 - negative binomial distribution and Wald test for significance, corrected for multiple testing with the Benjamini–Hochberg (BH) procedure; GO Enrichment - hypergeometric test and the BH procedure for multiple testing; GSEA - Kolmogorov–Smirnov-like statistic, significance assessed via permutation testing, and the standard cutoff is FDR < 0.25; mass spectrometry - Student’s t-test, two-tailed (p < 0.05).

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