Fig. 1: Lack of caspase 8 expression promotes metastatic disease. | Nature Communications

Fig. 1: Lack of caspase 8 expression promotes metastatic disease.

From: Lack of caspase 8 directs neuronal progenitor-like reprogramming and small cell lung cancer progression

Fig. 1: Lack of caspase 8 expression promotes metastatic disease.The alternative text for this image may have been generated using AI.

a Caspase 8 protein expression (log2) of SCLC patients (n = 112) and matched adjacent normal lung samples (n = 112)18 is plotted. b Caspase 8 expression transcripts per kilobase million (TPM) (log2 TPM+1) in lung adenocarcinoma (TCGA; LUAD, n = 48) and SCLC (n = 81) samples is shown. c Spearman correlation of CASP8 expression (FPKM) versus the chromosomal status of CASP8 determined as integral copy number (iCN) for n = 71 patients with SCLC20. d Methylation determined for 33 patient-matched tumor and normal samples by MeDIP-seq. CASP8 promoter methylation levels (average methylation of ten different regions) are plotted. e Pearson correlation determined for CASP8 methylation (CASP8 methylation mean region 4–8) and CASP8 NM_033356 expression (FPKM). f Expression levels (log2 TPM+1) of caspase 8 in human (n = 14) and mouse SCLC (n = 10), human (n = 77) and mouse NSCLC (n = 5) cell lines. g Tumor incidence of RP mice (n = 19) and RPC mice (n = 21) based on first detection of tumors using MRI imaging. h Representative MRI images and photos of lungs of RP mice (n = 26) and RPC mice (n = 24) sacrificed at humane endpoint. Quantification of the number of tumor nodules per lung based on macroscopical evaluation at time of death. i Representative pictures of liver metastasis at humane time point of RP mice (n = 24) and RPC mice (n = 16) and quantification of liver metastasis incidence at endpoint based on macroscopical evaluation. j Survival of mice at 40 weeks after adenoCre inhalation (RP n = 13 and RPC n = 17). k qPCR analysis on endpoint tumors from RP (n = 5) and RPC (n = 5) mice. Expression values are depicted as relative expression (DCt) to housekeeping gene. l Representative images of lung sections from mice with the indicated genotypes sacrificed at humane endpoint, stained with H&E or immunostained for Ki67, NCAM (CD56), CD31 or CD45 are shown. Scale bars: 100 μm (RP n = 6 and RPC n = 10). a, b, d, f paired t tests (two tailed). c Spearman correlation (two-tailed). e Pearson´s correlation (two tailed). g–j Fisher´s exact test (two-sided). Source Data are provided as a Source Data file.

Back to article page