Fig. 2: Absence of caspase 8 promotes reprogramming towards a neuronal progenitor-like state. | Nature Communications

Fig. 2: Absence of caspase 8 promotes reprogramming towards a neuronal progenitor-like state.

From: Lack of caspase 8 directs neuronal progenitor-like reprogramming and small cell lung cancer progression

Fig. 2: Absence of caspase 8 promotes reprogramming towards a neuronal progenitor-like state.The alternative text for this image may have been generated using AI.

a Uniform Manifold Approximation and Projection (UMAP) clustering of tumor cells (n = 28,000) from RP (n = 2 mice n = 7000 cells) and RPC (n = 2 mice, n = 7000 cells) samples. b Proportions of RP (yellow) and RPC (blue) cells within each cluster identified. c UMAP plot of RP (yellow) and RPC (blue) clusters. d Pathway enriched in STRING analysis on the upregulated cluster-defining genes are shown. Top enriched pathways within GO term ´Cellular Component´ plotted based on False Discovery Rate (FDR). e Volcano plot of differentially expressed genes (pseudo bulk) in RPC as compared to RP tumor cells. f Unsupervised pseudotime trajectory analysis of RP and RPC tumor cells (upper panel), density plots (dark colours=lower density) (lower panel). g Expression of SCLC-A/N/P/Y subtype genes on the trajectory. h Genes defining early (left) or late (right) pseudotime. i Heat map of pseudotime-defining gene clusters and sample distribution along pseudotime in density histograms. j Binary AUCell score activity projected onto RP (left) and RPC (right) UMAP space. k Number of RP (grey) and RPC (red) cells with binary AUCell score activity=on is plotted. Source Data are provided as a Source Data file.

Back to article page