Fig. 2: miR-71 targets dve-1 transcripts for degradation. | Nature Communications

Fig. 2: miR-71 targets dve-1 transcripts for degradation.

From: The microRNA miR-71 suppresses maladaptive UPRmt signaling through both cell-autonomous and cell-non-autonomous mechanisms

Fig. 2: miR-71 targets dve-1 transcripts for degradation.The alternative text for this image may have been generated using AI.

a-b, (a) representative photomicrographs of the heads of live animals (outlined by a white dashed line) and (b) quantification of mCherry fluorescence intensity of the his-72p::mCherryNLS::dve-1 3’UTR reporter. Scale bar, 20 μm. Columns represent mean ± SEM; n = 12; two-way Student’s t test. c schematic representation of the dve-1 3’UTR reporter annotated with the two putative sites and sequences (black boxes with white nucleotide sequences) of miR-71 seed sequence binding (gray sequences). These sites were mutated (orange sequences) to determine their relevance for miR-71-mediated regulation. d, e, (d) representative photomicrographs of the heads of live animals (outlined by a white dashed line) and (e) quantification of mCherry fluorescence intensity of the dve-1 3’UTR and dve-1 3’UTR** (seed recognition sites mutated) reporters. Scale bar, 20 μm. Columns represent mean ± SEM; n = 17, 20, 14 and 18; one-way ANOVA with Tukey’s post hoc test. f qPCR analysis of dve-1 mRNA levels. Columns represent mean ± SEM; n = 3 where each biological replicate is a population grown on a different plate; one-way ANOVA with Tukey’s post hoc test. Source data are provided as a Source Data file.

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