Fig. 3: miR-71 protects against maladaptive UPRmt hyperactivation.
a-d, (a) and (c) representative photomicrographs of animals (outlined by a white dashed line) and (b) and (d) quantification of GFP fluorescence intensity of the hsp-6p::GFP (n = 7 and 10) and hsp-60p::GFP reporters (n = 13 and 11). Scale bar, 50 μm. Bars represent mean ± SEM; two-way Student’s t test. e, f quantification of body wave initiation rate of L4 animals placed in liquid. Bars represent mean ± SEM. For e, n = 36, 100, 111 and 67; for f, n = 63, 37 and 42; one-way ANOVA with Tukey’s post hoc test. ATFS-1nuc is a constitutively nuclear-localized version of ATFS-1. g qPCR analysis of hsp-6 mRNA levels. Columns represent mean ± SEM; n = 3 where each biological replicate is a population grown on a different plate; one-way ANOVA with Tukey’s post hoc test. h quantification of body wave initiation rate of L4 animals placed in liquid. Bars represent mean ± SEM; n = 22, 49, 49 and 48; one-way ANOVA with Tukey’s post hoc test. i representative photomicrographs of body wall muscle tissue in L4 animals stained with phalloidin. Arrow heads indicate disruptions to actin filament structure. Scale bar, 10 μm. j quantification of actin filament appearance. Bars represent mean ± SEM; n = 11, 14, 14 and 17; one-way ANOVA with Tukey’s post hoc test. k quantification of body wave initiation rate of L4 animals placed in liquid. Bars represent mean ± SEM; n = 59; one-way ANOVA with Tukey’s post hoc test. For (e−k) WormLab automated software was used to quantify body wave initiation rates. Source data are provided as a Source Data file.
