Fig. 4: Structure of a DNA-bound XPF-ERCC1-SLX4IP-SLX4330-555 complex.
From: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways
a Cryo-EM map of the XPF-ERCC1-SLX4IP-SLX4330-555-DNA complex. XPF is shown in cyan, ERCC1 in blue, SLX4 in orange, SLX4IP in yellow, the scissile DNA strand in light green, and the non-scissile strand in dark green. b Atomic model of the XPF-ERCC1-SLX4IP-SLX4330-555-DNA complex (colours as in b). c Surface view of the proteins in the complex with residues in proximity to DNA (<3.5 Å) shown in pink. DNA is not shown. d Close-up view of the DNA contacts with the ERCC1 HhH2 domain. e Close-up view of the DNA contacts with the XPF nuclease and RecA1 domains. Manganese ions bound in the active site shown as spheres. f View of the XPF active site with two metal ions coordinated by negatively charged residues (shown in cyan) and a DNA backbone phosphate.
