Fig. 7: Biochemical analysis of the XPF-SLX4IP interface.
From: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways
a Locations of XPF mutations analysed (red spheres) on the structure. The 544-549 3AK mutant comprises the F544A, I546A, L547A, and E549K mutations in the domain-swapped β-strand of XPF. I554 and E572 were mutated individually and in combination with other mutations. b IP of wild-type and 544–549 3AK mutant eGFP-XPF from HEK293TN cells. 1% of input lysate to the IP was analysed as input. c Cis-platin survival assay using XPF knock-out cells complemented with eGFP-XPFWT and eGFP-XPF3AK mutant protein along with the parental strain and eGFP controls. Data from n = 3 biological repeats, each comprising 6 technical replicates, are displayed (as mean ± standard error of the mean). Relative metabolic activity in the absence of cis-platin was set to 100% for each cell line. Source Data are provided as a Source data file.
