Fig. 1: SLC35B2 KO reduces but does not completely inhibit SAFV-3 infection in HeLa-N cells. | Nature Communications

Fig. 1: SLC35B2 KO reduces but does not completely inhibit SAFV-3 infection in HeLa-N cells.

From: Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection

Fig. 1: SLC35B2 KO reduces but does not completely inhibit SAFV-3 infection in HeLa-N cells.The alternative text for this image may have been generated using AI.

a The graph displays the −log10-transformed robust ranking aggregation (RRA) scores of genes enriched following SAFV-3 infection in HeLa-N cells, analyzed using the MAGeCK software program. The X-axis represents data from the first CRISPR screen, while the Y-axis shows results from the second screen. The dotted line indicates the significance threshold of RRA = 0.01. Genes that met the criterion of RRA < 0.01 in both screens are highlighted in blue. The size of each dot reflects the combined enrichment across both screens, with larger dots indicating a greater sum of −log10 (RRA scores) from both experiments. b HS expression in HeLaN-∆SLC cells. Non-permeabilized cells were stained with an anti-HS antibody and analyzed using flow cytometry. c WT, HeLaN-∆SLC, and HeLaN-∆EXT1 cells were infected with tenfold serial dilutions of SAFV-3, and viable cells were stained with crystal violet to assess the infection levels. Images are representative of two independent experiments. d Multi-step growth kinetics of SAFV-3 in HeLaN-∆SLC and HeLaN-WT cells. The cells were infected with SAFV-3 and incubated for up to 5 days. Data are presented as mean viral titers with standard deviation (s.d.) (n = 3). Statistical significance was determined using the two-sided Welch’s t-test. **, P < 0.01. The dotted line indicates the limit of detection. Source data are provided as a Source Data file. Ctrl control.

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