Fig. 1: Single-cell transcriptome profiles of murine sclera from the form-deprived eyes and the fellow eyes.

a The schematics of form deprivation treatment, scleral single cell dissociation, and single cell RNA sequencing in 10× Genomics. b The UMAP plot shows 20 cell clusters. These clusters are further separated into 10 major cell types. c Violin plots show the log-transformed expression level of canonical markers in each cluster. Different colours are used to distinguish between the 10 major cell types. d The UMAP plot shows 16 subtypes of scleral fibroblasts. e The heat map shows the relative average expression of the most strongly enriched genes and the key genes in each subtype of scleral fibroblasts. UMAP uniform manifold approximation and projection.