Fig. 6: High-confidence in vivo interaction network of POLE2, revealed by proximity labeling mass spectrometry.
From: The non-catalytic DNA polymerase ε subunit is an NPF motif recognition protein
A Selected interaction partners of POLE2 are shown in dotplot with the node sizes adjusted based on measured relative abundances. Components of the ε polymerase complex, the partners that are consistently impacted by interface mutations and some reference interactors are shown of which measured abundance does not show change upon mutations. B AF3 prediction between POLE2 and POLA1 suggests a potential NPF motif-mediated interaction. C nHU experiment confirmed the interaction between the isolated NPF motif of POLA1 and POLE. Experiments were carried out with two technical replicates and the obtained standard deviations are displayed on the means for each measurement points to indicate range of measurements. D The Y513C variant that shows decreased stability in proximity labeling experiments localizes to granular cytoplasmic structures that are otherwise absent in cells expressing WT POLE2. Localization experiments were carried out in at least 3 independent experiment series. Source data can be found in Supplementary Data 4 and Supplementary Figs. 17, 18, and in the Source Data File.
