Fig. 4: BPTF promotes AR binding at promoters, enhancers and super-enhancers of AR target genes. | Nature Communications

Fig. 4: BPTF promotes AR binding at promoters, enhancers and super-enhancers of AR target genes.

From: BPTF regulates androgen receptor activity by enhancing chromatin accessibility and stabilizing the AR-FOXA1 interaction

Fig. 4: BPTF promotes AR binding at promoters, enhancers and super-enhancers of AR target genes.The alternative text for this image may have been generated using AI.

A Differential analysis of AR ChIP-seq peaks in BPTF-KD Rv1 cells. Peaks enriched in control and BPTF-KD cells are highlighted in red (p < 0.05). B Heatmap showing decreased AR peak signals in BPTF-KD cells; these peaks are defined as BPTF-dependent AR peaks. C Proportion of BPTF-dependent AR peaks localized at active enhancer and promoter regions. D Peak profile plots of BPTF-dependent AR peaks, showing AR (top) and BPTF (bottom) signal intensities at active enhancer and promoter regions after BPTF KD in Rv1 cells. E Track images showing BPTF and AR peaks at promoter and enhancer regions of representative AR target genes after BPTF KD in Rv1 cells. F ChIP-qPCR analysis of AR at the promoter or enhancer regions of representative AR target genes after BPTF KD in Rv1 cells. G BETA analysis showing the correlation between BPTF-dependent AR peaks (i.e., AR ChIP-seq peaks reduced upon BPTF KD) and BPTF-activated genes (i.e., transcripts downregulated in BPTF-KD RNA-seq). H GO analysis of direct BPTF-dependent AR target genes identified in (G). I Identification of super-enhancers (SEs) in Rv1 cells using the rank ordering of super-enhancers (ROSE) algorithm, based on enhancers defined by the H3K27ac/H3K4me1 ChIP-seq peaks. J Peak profile plots showing BPTF (left) and AR (right) signal intensities at SE sites after BPTF KD in Rv1 cells. K Track images showing BPTF and AR peaks at SEs of representative AR target genes after BPTF KD in Rv1 cells. L Dot plot showing the ssGSEA-derived scores for SE-associated genes (n = 80) based on BPTF-KD RNA-seq data. M GO analysis of the reduced SE-associated genes after BPTF KD in Rv1 cells. CUT&RUN ChIP-seq data (A–E, I–K) were generated from two biological replicates. Data are from three independent biological replicates (L) or are representative of three independent biological replicates (F). Data are presented as mean ± SD (F). Statistical significance was determined using a two-tailed unpaired Student’s t-test (F, L). Source data are provided as a Source data file.

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