Fig. 3: The loss of 4EHP affects the outcome of proteostasis phenotypes in degenerative disease models.

a–c The parkin (park) loss-of-function phenotype is suppressed by the loss of 4EHP. a Thor^intron-DsRed expression in one-day-old adult flies of the indicated genotypes. park^D21/park²⁵ flies have intense DsRed induction, which is partially suppressed in the 4EHP^CP53 homozygous background. b Quantification of the DsRed signal from flies shown in (a). One-way ANOVA and Tukey’s HSD were used to assess statistical significance. c The lifespan of the flies of the indicated genotypes. park^D21/park²⁵ flies show high levels of lethality immediately after eclosion, and very few flies survive more than 40 days. 4EHP^CP53 in that genetic background significantly enhanced survival. Log-rank (two-sided) was used to assess statistical significance. p < 0.0001 between control and park^D21/park²⁵. p < 0.0001 between park^D21/park²⁵ and park^D21, 4EHP^CP53/park²⁵, 4EHP^CP53. d Light-dependent retinal degeneration is accelerated in 4EHP^CP53 flies. Pseudopupils were used to assess retinal integrity in live flies. (Left) A graph showing the percentage of flies with intact pseudopupils when reared under light. 4EHP^CP53 flies (red line) exhibit accelerated retinal degeneration as compared to control (w¹¹¹⁸) flies (black line). crc^GFSTF flies (blue line) have early onset retinal degeneration. (Right) Flies with intact pseudopupils when reared in the dark. The log-rank test (two-sided) was used to assess statistical significance between all three genotypes in light conditions (left, p < 0.0001) and in dark conditions (right, p > 0.9999).