Fig. 1: MCARE is predominantly expressed in fast-twitch muscle and localizes to the SR. | Nature Communications

Fig. 1: MCARE is predominantly expressed in fast-twitch muscle and localizes to the SR.

From: MCARE enhances SERCA1 activity in fast-twitch muscle to maintain calcium handling and muscle integrity

Fig. 1: MCARE is predominantly expressed in fast-twitch muscle and localizes to the SR.

a Alignment of the amino acid sequence of MCARE from different species. The conservation rate of each amino acid is indicated at the top. b qRT-PCR showing relative mRNA expression of Mcare, SERCA isoforms, and MRLN across adult mouse tissues. N.D. not detected. n = 4 mice. Error bars represent mean ± SD. c Western blot of adult mouse tissues and C2C12 myoblasts (mb) and myotubes (mt) with MCARE- and GAPDH-specific antibodies. Each lane contains pooled lysates from three independent biological sources (tissues or wells). d Fluorescence microscopy images of C2C12 myoblasts expressing EGFP-tagged MCARE and mCherry-tagged SERCA1a. Scale bar, 10 μm. e Fluorescence microscopy images of isolated mouse TA muscle fibers after in vivo electroporation with EGFP-MCARE and mCherry-SERCA1a. Scale bar, 5 μm. f Western blot of whole skeletal muscle tissue and its corresponding microsomal fraction from a mouse, probed with the indicated antibodies. g Myc-SERCA1a and Flag-MCARE were transiently expressed in C2C12 myoblasts. Proteins were detected by immunofluorescence analysis in completely permeabilized (Triton X-100) and selectively permeabilized (digitonin) cells. Scale bar, 10 μm. Gastro gastrocnemius, Quad quadriceps, E. WAT epididymal white adipose tissue, BAT brown adipose tissue.

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