Fig. 3: MCARE displaces MRLN and enhances SERCA activity. | Nature Communications

Fig. 3: MCARE displaces MRLN and enhances SERCA activity.

From: MCARE enhances SERCA1 activity in fast-twitch muscle to maintain calcium handling and muscle integrity

Fig. 3: MCARE displaces MRLN and enhances SERCA activity.

a Western blot of 5% input and immunoprecipitated (IP) Flag fractions from HEK293 cells transfected with Flag-MCARE and Myc-SERCA1a. b Protein docking simulation using ClusPro (https://cluspro.org) predicted that MCARE and MRLN occupy the same groove in SERCA1a. c Western blot of 5% input lysates and IP Flag fractions from HEK293 cells transfected with Flag-SERCA1a, EGFP-MRLN, and MCARE. d SERCA activity and affinity for Ca2+ (Kca) were measured in membrane vesicles from HEK293 cells co-transfected with SERCA1a and the indicated constructs. n = 4 biological replicates. Error bars represent mean ± SD. Statistical significance was assessed using two-way ANOVA with Tukey’s multiple comparisons test.

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