Fig. 1: Low cell number mass spectrometry-based proteomic workflows.
From: Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma
A Schematic showing the mini-bulk and single cell proteomic (SCP) experimental design. Neutrophils (CD45+, CD66b+, CD49d−) were sorted using flow cytometry directly into 384 well plates containing the master mix, later processed on the cellenONE X1 and analyzed on the Orbitrap Astral. B Schematic showing the number of cells that are analyzed in bulk, mini-bulk and single cell proteomics. C Violin and box plot showing the number of proteins identified in bulk (n = 15), mini-bulk (n = 29) and single cell proteomics (n = 277). D Percentage of proteins identified with less than 5500 copies (median copy number in bulk) across all 3 proteomic workflows. E Dot plot showing proteins identified in mini-bulk and SCP ordered by their median copy number in bulk. Chord diagrams showing the coverage of F granule proteins, G metabolic proteins and H immune signalling proteins across bulk, mini-bulk and single cell proteomics. For all chord diagrams the ribbons are sized by median copy number of each protein. For all boxplots, the top and bottom hinges represent the 1st and 3rd quartiles. The top whisker extends from the hinge to the largest value no further than 1.5× interquartile range (IQR) from the hinge; the bottom whisker extends from the hinge to the smallest value at most 1.5× IQR of the hinge. .
