Fig. 4: Single cell proteomic analysis of TANs identified discrete functional clusters.
From: Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma
A Uniform manifold approximation and projection (UMAP) showing the tumour associated neutrophil (TAN) clusters. (n = 277). B Sankey diagram showing the proportions represented by each cluster. C Dotplot showing maturity marker proteins across all clusters. D Heatmap showing mitochondrial metabolism and mitochondrial membrane proteins across all cells ordered by cluster. E Boxplot showing the summed normalised intensity of all ribosomal proteins. F Dotplot showing complement and coagulation proteins across all clusters. Boxplots showing the summed normalised intensity of all G azurophilic granules, H histones and I nuclear membrane proteins across all clusters. Armed (Arm. n = 76), Engaged (Eng. n = 46), Vital NETs (VN, n = 33), Exhausted (Exh. n = 47), Lytic NETs (LN, n = 32), Immunosuppressive and angiogenic (I&A, n = 23) and Vascular Immature (VI, n = 22). For all boxplots the top and bottom hinges represent the 1st and 3rd quartiles. The top whisker extends from the hinge to the largest value no further than 1.5× interquartile range (IQR) from the hinge; the bottom whisker extends from the hinge to the smallest value at most 1.5× IQR of the hinge. For panels (G–I) all protein family boxplots p-values were calculated using a two-sided Welchs T test.
