Fig. 3: Oleic acid content association with PeanutMAGIC pangenomic and single reference markers.

a Manhattan plot showing the association signals of pangenome-based markers with oleic acid content from general linear model genome wide association study. A Bonferroni-corrected P value of 0.05 was used as a significant threshold (P = 1.1 × 10−7), represented by a horizontal dashed red line. b Local association plot of Chr.19 from (a) showing multiple significant locations. The orange highlight represents the location of the functional gene AhFAD2B. Regions upstream of a known functional gene have higher association values. c Genetic view of the associated region showing significant markers and genes of interest. Markers are highlighted in green and denoted above the genome bar with P values and physical locations. The known functional gene AhFAD2B is highlighted orange. Between the associated markers and a known functional gene, a third FAD2 gene was identified and termed AhFAD2C, highlighted in yellow. d Manhattan plot showing PeanutMAGIC Core single-reference marker signals associated with oleic acid content from general linear model genome wide association study, with an additional signal identified on Chr.05. e Local association plot on Chr.19 from (d) showing a strong association downstream of the known AhFAD2B location, with no signal observed at the physical position of AhFAD2B. Source data are provided as a Source Data file.