Fig. 3: DNA-PK bound to mono-nucleosomes.
From: Cryo-EM structures of NHEJ assemblies with nucleosomes
A DNA-PK with 153 bp H2AX nucleosome model/map 1 (to 4.5 Å resolution), showing DNA-PKcs and Ku70/80 bound to nucleosome DNA. The nucleosome is positioned close to Ku70 and Ku80 vWA is closed. Ku80 vWA coloured in lime and Ku70 vWA in salmon. B DNA-PK with 153 bp H2AX nucleosome model/map 2 (to 4.2 Å resolution), showing DNA-PKcs and Ku70/80 bound to nucleosome DNA. The nucleosome is positioned closer to Ku80 and Ku80 vWA is open. Ku80 vWA coloured in lime and Ku70 vWA in salmon. C Ku80-mediated DNA-PK dimer to 4.4 Å resolution, with two nucleosomes held by the formation of the DNA-PK dimer. D DNA-PK model from A, but showing a local filtered map with the contour level high, indicating the presence of extra density for an additional Ku70/80 molecule near the DNA-PKcs kinase active site and an additional Ku70/80 at the other side of the nucleosome. Ku70 is shown in orange, Ku80 in green, DNA in dark grey, DNA-PKcs in light grey and histone coloured according to the key.
