Fig. 4: DNA-PK bound to nucleosomes following the addition of AMP-PNP.
From: Cryo-EM structures of NHEJ assemblies with nucleosomes
A Representative cryo-EM micrograph (from 12,898 micrographs total), scale bar 20 µm, with DNA-PK nucleosome filaments highlighted with a red box. B Representative 2D classes, scale bar 340 Å. C Cryo-EM map and model for DNA-PK with 153 bp H2AX nucleosome following the addition of AMP-PNP to 5 Å resolution. DNA-PKcs is shown in light grey, DNA in dark grey, Ku70 in orange, Ku80 in green and the histones coloured according to the key. D Model of an extended filament of DNA-PK bound to nucleosomes. Ku70 is shown in orange, Ku80 in green, DNA as a dark grey surface, DNA-PKcs in light grey and the histones in black.
