Fig. 1: nPME is located in the symbiosome space. | Nature Communications

Fig. 1: nPME is located in the symbiosome space.

From: Symbiosome functionality in Medicago truncatula nodules requires continuous clearing of pectins from the symbiosome space

Fig. 1: nPME is located in the symbiosome space.

Confocal images of 14 days old nodules embedded in agarose and sectioned. a–a” nPME is located within symbiosomes in infected cells (IC) of the nodule interzone (n = 20 nodules). b Fluorescence intensities were measured along the region marked with white line and white arrow in (a”). Fluorescence intensity measurements were performed with Fiji, and graphs were generated with GraphPad Prism 10. c, d The localization of nPME-GFP is shown as a 3D projection generated by using Imaris (c) and the sketch was drawn using Adobe Illustrator (d). e–e” nPME does not accumulate along nodular ITs (n = 20 nodules). The experiment was conducted with 3 independent replicates, each involving analysis of at least 20 nodules. Green indicates the GFP-tagged nPME signal and magenta indicates the mCherry-tagged S. meliloti 2011. Scale bars = 10 μm.

Back to article page