Fig. 2: Recognition of dsRNA by J2.
From: Structural basis of double-stranded RNA recognition by the J2 monoclonal antibody
a Front, side, and rear views of the J2 Fab-dsRNA complex structure, showing two Fabs bound to opposite sides of the dsRNA. Heavy chain (HC) is in blue; light chain (LC) in orange. VH and CH1: variable and first constant domains of the heavy chain; VL and CL: variable and constant domains of the light chain. b, c Closed (b) and open-book (c) views of J2 Fab-dsRNA interfaces colored by solvent-accessible areas buried per residue (Å2). d Overlay of the two crystallographically observed J2 Fab-dsRNA complexes, colored as in (a). e Overlay of Fab-dsRNA complex structure colored as in (a) with an idealized 23-bp dsRNA (pink). f Diagram of secondary structures and interactions of each J2 Fab with the dsRNA. Nucleotides in direct polar contacts are in a darker red color. J2 footprint on the dsRNA is shown in a green background. Proposed hydrogen bonds are shown as dashed magenta lines with arrowheads; π -π stacking interaction as parallel magenta lines. RNA 2′-hydroxyls in contact with J2 are shown as red spheres. Complementarity-determining regions (CDRs) H1-H3 and L1-L3 are denoted.
