Fig. 3: Detailed J2 – dsRNA interfaces.
From: Structural basis of double-stranded RNA recognition by the J2 monoclonal antibody
a, b Detailed interfaces and contacts between the dsRNA (red and salmon, for chains E and F, respectively) and J2 heavy chain CDRs ((a), HC, blue) and light chain CDRs ((b), LC, orange). Proposed hydrogen bonds and π-π interactions are shown as green and cyan dashes, respectively. For clarity, the 310 helix in CDR-H1 is shown in cartoon loop instead of helical mode and denoted. c Representative BLI sensorgrams for a 20-bp dsRNA binding to WT, HC, and LC mutant J2 IgGs at 100 mM KCl. d Summary of the data in (c), showing the maximum sensor responses (nm). Values are mean ± s.d. of three biologically independent samples.
