Fig. 4: Effects of nucleotide composition on J2 binding.
From: Structural basis of double-stranded RNA recognition by the J2 monoclonal antibody
a Nine 30-bp dsRNAs with GC contents ranging from 0% to 100% used for J2 IgG binding analyses. G-C pairs are indicated in red; A-U pairs in black. b Competition FP titrations of dsRNAs in (a) against 3′-FAM labeled VA-I RNA prebound to J2 IgG. IC50s are indicated. c FP-derived IC50s derived from (b) (top) and BLI maximum sensor responses from (d) (bottom) for the dsRNAs in (a). Asterisks: no detectable binding. d Representative BLI sensorgrams (top, at 200 mM KCl), temperature-scanning CD spectra (middle) and unfolding transitions (lower) of dsRNAs in (a) binding to J2 IgG. Different shades of blue indicate temperature transitions from 20 °C (dark blue) to 97 °C (light blue). e Comparison of the CD spectra of dsRNAs in (a) at 20 °C. f Melting temperatures (Tms) of dsRNAs in (a) derived from temperature-scanning CD data in (d). Asterisks: Tm > 85 °C and out of measurable range. Values are mean ± s.d. of three biologically independent samples.
