Fig. 5: Effects of homotypic repeat sequences and nucleotide skew on J2 binding.
From: Structural basis of double-stranded RNA recognition by the J2 monoclonal antibody
a Sequences of dsRNA, dsDNA, RNA-DNA hybrids, ssRNA, and ssDNA of repetitive sequences (40 bp or 40 nts) used in J2 IgG binding analyses. b Representative BLI sensorgrams (top, at 400 mM KCl), temperature-scanning CD spectra (middle) and unfolding transitions (lower) of duplex nucleic acids in (a) binding to J2 IgG. Different shades of blue indicate temperature transitions from 20 °C (dark blue) to 97 °C (light blue). c Representative BLI sensorgrams (top, at 400 mM KCl) and temperature-scanning CD spectra (bottom) of single-stranded nucleic acids in (a) binding to J2 IgG. d Comparison of the CD spectra of nucleic acids in (b, c) at 20 °C. e Melting temperatures of duplex nucleic acids in (a) derived from temperature-scanning CD data in (b).
