Fig. 1: Genome-scale tiling CRISPRi-based genetic screens identify hDels modifying cellular proliferation.
From: Mapping cis- and trans-regulatory target genes of human-specific deletions
a Great ape cladogram. The number of deletions assigned to the human lineage1 and the number of base pairs removed are labeled. b CRISPRi-based tiling of hDels (hDel-v1). hDel-v1 sgRNAs were selected from 50-bp genomic windows. c hDel-v1 screening approach in chimpanzee iPS cells. d Scatterplot of sgRNA log2 fold-change for hDel-v1 technical replicates in C3624K. e Volcano plot of hDel-targeting and non-targeting sgRNA log2 fold-change and DESeq2 Benjamini–Hochberg-adjusted p-value. f Manhattan plot of hDel position in the chimpanzee reference genome (panTro6) and α-RRA Benjamini–Hochberg-adjusted p-value for 500-bp hDel genomic windows (gold, FDR < 0.1). g hDel_6304-targeting sgRNA log2 fold-change (gold, FDR < 0.05) and MBD3 Omni ATAC-seq, H3K4me1, H3K4me3, and H3K27ac in C3624K. Data are the mean of two technical replicates ± standard error.
