Fig. 2: High-density tiling CRISPRi screen refines the boundaries of functional sequences within proliferation-modifying hDels.
From: Mapping cis- and trans-regulatory target genes of human-specific deletions
a High-density CRISPRi-based tiling of hDels (hDel-v2). b Distribution of the distance between adjacent hDel-targeting sgRNAs (top) and the number of sgRNAs per hDel (bottom) for hDel-v1 and hDel-v2. c Scatterplot of sgRNA log2 fold-change for hDel-targeting sgRNAs screened in hDel-v1 and hDel-v2 (n = 18,148 sgRNAs). d Identification of proliferation-modifying hDels. 250-bp hDel genomic windows are ranked by α-RRA Benjamini–Hochberg-adjusted p-value (gold, FDR < 0.1). e Upset plot of Omni-ATAC seq, H3K4me1, H3K4me3, H3K27ac, and H3K27me3 intersecting hDels (FDR < 0.1) in C3624K. f hDel_7051-targeting sgRNA log2 fold-change (gold, FDR < 0.05) and ATRX Omni-ATAC seq, H3K4me1, H3K4me3, and H3K27ac in C3624K. Data are the mean of two technical replicates ± standard error.
