Fig. 5: hDel_2247 regulates PLPP1 and drives lacZ expression in developing anterior cortex and olfactory bulb.
From: Mapping cis- and trans-regulatory target genes of human-specific deletions
a hDel_2247-targeting sgRNA log2 fold-change (hDel-v1) and Omni ATAC-seq, H3K4me1, H3K4me3, and H3K27ac in C3624K. Data are the mean of two technical replicates ± standard error. b Differential PLPP1 expression for cells (“pseudobulk”) harboring the indicated hDel_2247-targeting sgRNA (*FDR < 0.1). Data are the mean of five technical replicates (GEM wells) ± standard error. c Differential PLPP1 expression (qRT-PCR) for cells edited with hDel_2247-targeting Cas9 RNPs (*FDR < 0.05). Bars represent independent polyclonal populations of chimpanzee iPS cells (C8861). Data are the mean of four technical replicates ± standard error. d Representative E13.5 hDel_2247::lacZ mouse embryo stained for β-galactosidase (LacZ) activity (n = 5 embryos). e Sagittal section showing lacZ expression in olfactory bulb and anterior cortex. Ctx cortex, OB olfactory bulb, SE septum, P posterior, A anterior.
