Fig. 2: VPS13B required for mitochondrial membrane potential and membrane dynamics.
From: VPS13B recruits lipid vesicles to promote mitochondrial fission and quality control
A Live-cell images of WT and KO cells transfected with VPS13B‑FL‑EGFP and VPS13B‑ΔMLD‑EGFP stained with TMRM and Mitotracker DeepRed. Scale bars, 10 µm. B Quantification of TMRM intensity normalized to Mitotracker area. Analysis via one-way ANOVA with Dunnett’s test.***p < 0.001, *p = 0.01, n.s. = not significant. WT; n = 34, KO; n = 57, KO + FL; n = 28, KO + ΔMLD; n = 43 cells. All data are obtained from 3 independent experiments. C Confocal images from Fluorescence Recovery After Photobleaching (FRAP) assays of WT and KO cells transfected with VPS13B‑FL‑EGFP and VPS13B‑ΔMLD‑EGFP and mRFP-TOM20. Scale bars, 10 µm. D Quantitative analysis of fluorescence recovery. Analysis via Kruskal-Wallis with Dunn’s test. Data are presented as mean ± SEM. WT vs. KO1, p = 0.0451, KO1 vs. KO1 + FL, p = 0.0321; KO1 + FL vs. KO1 + ΔMLD, p = 0.0003. WT; n = 20, KO1; n = 13, KO1 + FL; n = 22, KO1 + ΔMLD; n = 21 mitochondrial region of interests(ROIs). All data are obtained from 3 independent experiments. E Half-time of recovery (t1/2) of mRFP-TOM20 calculated from one-phase association fits. Statistical significance between groups was determined by the Extra sum-of-squares F test. Cl: Confidence Interval. Data are presented as mean ± SEM.
