Fig. 5: VPS13B contacts mitochondrial fission sites with Drp1.
From: VPS13B recruits lipid vesicles to promote mitochondrial fission and quality control
A Confocal images of WT cells transfected with EGFP-Drp1, stained with VPS13B antibodies and Mitotracker. B Line scan analysis showing relative fluorescence intensity from each labeled protein (Green line: Drp1, Gray line: Mitotracker, Magenta line: VPS13B). Scale bars, 5 µm. C Live cell imaging of KO cells transfected with BFP-Drp1, VPS13B‑FL‑EGFP or VPS13B‑ΔMLD‑EGFP and stained with MitoTracker. Arrowheads point to the mitochondria contact site, and arrows point to the mitochondria fission site. Scale bars, 2 µm. D–G Quantification of mitochondria fission events in contact D, mitochondrial fission events in contact with Drp1 E, VPS13B F, and both Drp1 and VPS13B G. Analysis via two-tailed Mann-Whitney test. D **p = 0.002, E ***p < 0.001, F ***p = 0.001, G **p = 0.002, KO1 + FL; n = 9, KO1 + ΔMLD; n = 11 cells. All data are obtained from 3 independent experiments. Data are presented as mean ± SEM.
