Fig. 7: ZIKV CC_FLE sE stimulates nAb responses in OmniMouse animals expressing naïve human Ig loci, with a substantial portion focused on ZIKV E dimer-specific epitopes.

A Immunization schedule. Two female OmniMouse mice, OM5.1 and OM5.2, aged 20–21 weeks, were immunized with CC_FLE sE via subcutaneous (SC) route or intraperitoneal (IP) route at indicated time points. B Immune sera neutralization specificity mapping by neutralization depletion with various sE-based antigens (sE Ags). Terminal sera were pre-mixed with sE variant antigens to deplete cognate serum neutralizing antibodies before ZIKV H/PF/2013 RVP incubation, with mAbs, ZV67 (DIII specific) and EDE1-C8 (dimer context specific) as controls. Mock refers to medium containing no antigen added to immune sera or mAb for comparison. C CC_FLE sE reactive memory B cell sorting using splenocytes from animal OM5.1. SA-PE, Streptavidin-Phycoerythrin conjugate. D ELISA binding profiles of selected CC_FLE-elicited mAbs. Reference mAbs include EDE1-C8 (EDE-specific) and Z004 (DIII-specific). E Epitope clustering by BLI-based Ab competition assay. Left: assay scheme; Right: relative CC_FLE sE binding signal after co-incubation of test mAb and competitor mAbs. F ZIKV H/PF/2013 RVP, and G Authentic ZIKV FSS13025 neutralization mediated by mAbs, assessed by PRNT. In the assays in (B, D, F, G), each sample test was duplicated. In (B, D, F), each assay was repeated at least two times. Source data are provided as a Source Data file.