Fig. 9: OZ-D4 HC and LC gene amino acid sequence and clonal affinity maturation. | Nature Communications

Fig. 9: OZ-D4 HC and LC gene amino acid sequence and clonal affinity maturation.

From: Rational design of flavivirus E protein vaccine optimizes immunogenicity and mitigates antibody dependent enhancement risk

Fig. 9: OZ-D4 HC and LC gene amino acid sequence and clonal affinity maturation.

A Alignment of mature OZ-D4 HC (OZ-D4_H) and LC (OZ-D4_L) and the corresponding inferred germline (GL) human Ig V(D)J gene segment sequences. SHM, somatic hypermutation level by amino acid sequence. Divergent residues between the mature OZ-D4 and OZ-D4_GL are in red font. Residues involved in contacting ZIKV sE dimer are denoted with magenta circles. CDRs are delineated using the Kabat system. B Kinetics of mature IgG OZ-D4 and the inferred germline version (naïve precursor), OZ-D4_GL binding to ZIKV sE antigens (Ags) analyzed by Bio-Layer Interferometry (BLI). Left: BLI binding curves of OZ-D4 and OZ-D4-GL IgG1 molecules; Right: dissociation constant (KD), on-rate (kon), and off-rate (koff) values for each ZIKV sE antigen. nb, no binding. Antibodies were initially captured on anti-human Fc BLI probe, followed by immersing into wells containing sE ranging from 31.3 to 250 nM in concentration. C Neutralization activity of OZ-D4 and OZ-D4-GL against ZIKV H/PF/2013 RVP. Each sample test was duplicated, and each assay was repeated at least two times. Source data are provided as a Source Data file.

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