Fig. 5: Compressive force activates IKKβ and NF-κB via PIEZO1. | Nature Communications

Fig. 5: Compressive force activates IKKβ and NF-κB via PIEZO1.

From: Compression-induced NF-κB activation sustains tumor cell survival in confinement by detoxifying aldehydes and promotes metastasis

Fig. 5: Compressive force activates IKKβ and NF-κB via PIEZO1.

a A549 cells under confinement or unconfinement were analyzed by immunoblotting. The samples derive from the same experiment but different gels for IKKβ, IKBα, and another for IKKβ pS177/181, IKBα pS32/36, Actin were processed in parallel. b A549 cells were treated with or without compression at different time and analyzed by immunoblotting. The samples derive from the same experiment, but different gels for IKKβ, IKBα, another for IKKβ pS177/181, IKBα pS32/36, and another for Actin were processed in parallel. c A549 cells were treated with or without compression at different time. The cytosolic and nuclear fractions were prepared and analyzed by immunoblotting. The samples derive from the same experiment but different gels for RelA and another for LaminB1, Tubulin were processed in parallel. d A549 cells transfected with NF-κB-Luc reporter were treated with or without compression for 2 h. Relative luciferase activity was normalized to controls. e A549 cells with or without IKKβ depletion were treated with or without compression. Immunoblotting analyses were performed. The samples derive from the same experiment, but different gels for IKBα, another for IKBα pS32/36 and another for Actin were processed in parallel. f, g A549 cells with or without IKKβ depletion were treated with or without compression for 6 h. ALDH1B1 mRNA levels were quantified (f). ALDH1B1 protein levels were detected (g). h A549 cells with or without PIEZO1 depletion were treated with or without compression. Immunoblotting analyses were performed. The samples derive from the same experiment, but different gels for IKKβ, IKBα, another for IKKβ pS177/181, IKBα pS32/36, and another for Actin were processed in parallel. Data are presented as mean ± SD (n = 3 biologically independent experiments) (d, f). Immunoblotting experiments were performed with the indicated antibodies. Data are representative of three independent experiments (a–c, e, g, h). P-values were calculated using unpaired two-tailed Student’s t test (d, f). NS not significant, Rel. relative. Source data are provided as a Source Data file.

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